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crispr tgr5 knockout  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology crispr tgr5 knockout
    Crispr Tgr5 Knockout, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgr5+crispr/tgr5+sirna/pm32961266-107-0-6
    Average 90 stars, based on 1 article reviews
    crispr tgr5 knockout - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    CRISPR:

    Article Title: Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCε/ALDH2 pathway after subarachnoid hemorrhage in rats.
    Article Snippet: .. TGR5 CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA), ALDH2 CRISPR (Santa Cruz Biotechnology, Dallas, TX, MA, USA), or scrambled CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA) was injected into the ipsilateral (left) ventricle at 48 h before SAH. ..

    Article Title: Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCε/ALDH2 pathway after subarachnoid hemorrhage in rats.
    Article Snippet: .. TGR5 CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA), ALDH2 CRISPR (Santa Cruz Biotechnology, Dallas, TX, MA, USA), or scrambled CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA) was injected into the ipsilateral (left) ventricle at 48 h before SAH. ..

    Injection:

    Article Title: Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCε/ALDH2 pathway after subarachnoid hemorrhage in rats.
    Article Snippet: .. TGR5 CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA), ALDH2 CRISPR (Santa Cruz Biotechnology, Dallas, TX, MA, USA), or scrambled CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA) was injected into the ipsilateral (left) ventricle at 48 h before SAH. ..

    Article Title: Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCε/ALDH2 pathway after subarachnoid hemorrhage in rats.
    Article Snippet: .. TGR5 CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA), ALDH2 CRISPR (Santa Cruz Biotechnology, Dallas, TX, MA, USA), or scrambled CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA) was injected into the ipsilateral (left) ventricle at 48 h before SAH. ..



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    Representative western blot bands of time course (A) and densitometric quantification of <t>TGR5</t> (B) in the ipsilateral hemisphere after SAH. Data was represented as mean ± SD. n=6 per group. *P<0.05 vs. Sham group; One-way ANOVA, Tukey’s post hoc test. (C) Representative microphotographs of co-immunofluorescence staining of TGR5 (red) with neurons (NeuN, green), astrocytes (GFAP, green), and microglia (Iba-1, green) in the ipsilateral basal cortex at 24 h after SAH. Nuclei were stained with DAPI (blue). A small black square within coronal section of brain indicated the location of where the immunofluorescence staining images were taken. n=2 per group.
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    Representative western blot bands of time course (A) and densitometric quantification of <t>TGR5</t> (B) in the ipsilateral hemisphere after SAH. Data was represented as mean ± SD. n=6 per group. *P<0.05 vs. Sham group; One-way ANOVA, Tukey’s post hoc test. (C) Representative microphotographs of co-immunofluorescence staining of TGR5 (red) with neurons (NeuN, green), astrocytes (GFAP, green), and microglia (Iba-1, green) in the ipsilateral basal cortex at 24 h after SAH. Nuclei were stained with DAPI (blue). A small black square within coronal section of brain indicated the location of where the immunofluorescence staining images were taken. n=2 per group.
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    Image Search Results


    Representative western blot bands of time course (A) and densitometric quantification of TGR5 (B) in the ipsilateral hemisphere after SAH. Data was represented as mean ± SD. n=6 per group. *P<0.05 vs. Sham group; One-way ANOVA, Tukey’s post hoc test. (C) Representative microphotographs of co-immunofluorescence staining of TGR5 (red) with neurons (NeuN, green), astrocytes (GFAP, green), and microglia (Iba-1, green) in the ipsilateral basal cortex at 24 h after SAH. Nuclei were stained with DAPI (blue). A small black square within coronal section of brain indicated the location of where the immunofluorescence staining images were taken. n=2 per group.

    Journal: Free radical biology & medicine

    Article Title: Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCε/ALDH2 pathway after subarachnoid hemorrhage in rats.

    doi: 10.1016/j.freeradbiomed.2019.09.002

    Figure Lengend Snippet: Representative western blot bands of time course (A) and densitometric quantification of TGR5 (B) in the ipsilateral hemisphere after SAH. Data was represented as mean ± SD. n=6 per group. *P<0.05 vs. Sham group; One-way ANOVA, Tukey’s post hoc test. (C) Representative microphotographs of co-immunofluorescence staining of TGR5 (red) with neurons (NeuN, green), astrocytes (GFAP, green), and microglia (Iba-1, green) in the ipsilateral basal cortex at 24 h after SAH. Nuclei were stained with DAPI (blue). A small black square within coronal section of brain indicated the location of where the immunofluorescence staining images were taken. n=2 per group.

    Article Snippet: TGR5 CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA), ALDH2 CRISPR (Santa Cruz Biotechnology, Dallas, TX, MA, USA), or scrambled CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA) was injected into the ipsilateral (left) ventricle at 48 h before SAH.

    Techniques: Western Blot, Immunofluorescence, Staining

    (A-B) TGR5 CRISPR and ALDH2 CRISPR pretreatment reversed the neurobehavioral benefits of INT-777 at 24 h after SAH, n=6 per group. (C-E) Representative western blot bands and densitometric quantification of TGR5 and ALDH2 demonstrated the efficacy of CRISPR knockout in naive and SAH rats, n=4 per group. Vehicle: 10% dimethyl sulfide. Data was represented as mean ± SD. *P<0.05 vs. Sham group; @P<0.05 vs. SAH+Vehicle group; &P<0.05 vs. SAH+scr CRISPR group; #P<0.05 vs. SAH+scr CRISPR group; One-way ANOVA, Tukey’s post hoc test; scr CRISPR, scrambled CRISPR; KO CRISPR, Knockout CRISPR.

    Journal: Free radical biology & medicine

    Article Title: Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCε/ALDH2 pathway after subarachnoid hemorrhage in rats.

    doi: 10.1016/j.freeradbiomed.2019.09.002

    Figure Lengend Snippet: (A-B) TGR5 CRISPR and ALDH2 CRISPR pretreatment reversed the neurobehavioral benefits of INT-777 at 24 h after SAH, n=6 per group. (C-E) Representative western blot bands and densitometric quantification of TGR5 and ALDH2 demonstrated the efficacy of CRISPR knockout in naive and SAH rats, n=4 per group. Vehicle: 10% dimethyl sulfide. Data was represented as mean ± SD. *P<0.05 vs. Sham group; @P<0.05 vs. SAH+Vehicle group; &P<0.05 vs. SAH+scr CRISPR group; #P<0.05 vs. SAH+scr CRISPR group; One-way ANOVA, Tukey’s post hoc test; scr CRISPR, scrambled CRISPR; KO CRISPR, Knockout CRISPR.

    Article Snippet: TGR5 CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA), ALDH2 CRISPR (Santa Cruz Biotechnology, Dallas, TX, MA, USA), or scrambled CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA) was injected into the ipsilateral (left) ventricle at 48 h before SAH.

    Techniques: CRISPR, Western Blot, Knock-Out

    (A) Representative western blot bands. (B-J) Densitometric quantification of TGR5, cAMP, p-PKCε/PKCε, ALDH2, HO-1, 4-HNE, Bcl-2, Bax, and Cleaved Caspase-3 (CC3) in the ipsilateral hemisphere at 24 h after SAH. Vehicle: 10% dimethyl sulfide. Data was represented as mean ± SD. *P<0.05 vs. Sham group, @P<0.05 vs. SAH+Vehicle group, &P<0.05 vs. SAH+scr CRISPR group; One-way ANOVA, Tukey’s post hoc test, n=6 per group; scr CRISPR, scrambled CRISPR.

    Journal: Free radical biology & medicine

    Article Title: Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCε/ALDH2 pathway after subarachnoid hemorrhage in rats.

    doi: 10.1016/j.freeradbiomed.2019.09.002

    Figure Lengend Snippet: (A) Representative western blot bands. (B-J) Densitometric quantification of TGR5, cAMP, p-PKCε/PKCε, ALDH2, HO-1, 4-HNE, Bcl-2, Bax, and Cleaved Caspase-3 (CC3) in the ipsilateral hemisphere at 24 h after SAH. Vehicle: 10% dimethyl sulfide. Data was represented as mean ± SD. *P<0.05 vs. Sham group, @P<0.05 vs. SAH+Vehicle group, &P<0.05 vs. SAH+scr CRISPR group; One-way ANOVA, Tukey’s post hoc test, n=6 per group; scr CRISPR, scrambled CRISPR.

    Article Snippet: TGR5 CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA), ALDH2 CRISPR (Santa Cruz Biotechnology, Dallas, TX, MA, USA), or scrambled CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA) was injected into the ipsilateral (left) ventricle at 48 h before SAH.

    Techniques: Western Blot, CRISPR

    (A) Representative western blot bands. (B-J) Densitometric quantification of TGR5, cAMP, p-PKCε/PKCε, ALDH2, HO-1, 4-HNE, Bcl-2, Bax, and Cleaved Caspase-3 (CC3) in the ipsilateral hemisphere at 24 h after SAH. Vehicle: 10% dimethyl sulfide. Data was represented as mean ± SD. *P<0.05 vs. Sham group, @P<0.05 vs. SAH+Vehicle group, &P<0.05 vs. SAH+scr CRISPR group; One-way ANOVA, Tukey’s post hoc test, n=6 per group; scr CRISPR, scrambled CRISPR.

    Journal: Free radical biology & medicine

    Article Title: Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCε/ALDH2 pathway after subarachnoid hemorrhage in rats.

    doi: 10.1016/j.freeradbiomed.2019.09.002

    Figure Lengend Snippet: (A) Representative western blot bands. (B-J) Densitometric quantification of TGR5, cAMP, p-PKCε/PKCε, ALDH2, HO-1, 4-HNE, Bcl-2, Bax, and Cleaved Caspase-3 (CC3) in the ipsilateral hemisphere at 24 h after SAH. Vehicle: 10% dimethyl sulfide. Data was represented as mean ± SD. *P<0.05 vs. Sham group, @P<0.05 vs. SAH+Vehicle group, &P<0.05 vs. SAH+scr CRISPR group; One-way ANOVA, Tukey’s post hoc test, n=6 per group; scr CRISPR, scrambled CRISPR.

    Article Snippet: TGR5 CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA), ALDH2 CRISPR (Santa Cruz Biotechnology, Dallas, TX, MA, USA), or scrambled CRISPR (Santa Cruz Biotechnology, Dallas, TX, USA) was injected into the ipsilateral (left) ventricle at 48 h before SAH.

    Techniques: Western Blot, CRISPR